Professor John D. Buynak

 

John D. Buynak

    

Professor

 Office: 311 Fondren Science
Department of Chemistry Phone: (214) 768-2484
Southern Methodist University Fax: (214) 768-4089
PO Box 750314    e-mail:
Dallas, TX 75275-0314    
  | Prof. Buynak's Home Page |    

Education & Experience:

  • NIH Postdoctoral Fellow, Yale University, 1980-1981

  • Ph.D. Rice University, 1980

Research Interests:

Bioorganic chemistry, medicinal chemistry, design of enzyme inhibitors, synthesis of beta-lactam antibiotics and beta-lactamase inhibitors.

Selected Publications:

Ganta, S. R.; Perumal, S.; Pagadala, S. R. R.; Samuelsen, O.; Spencer, J.; Pratt, R. F.; Buynak, J. D. “Approaches to the simultaneous inactivation of metallo- and serine-b-lactamases” Bioorg. Med. Chem. Lett. 2009, 19, 1618-1622.

Kalp, M.; Totir, M. A.; Buynak, J. D.; Carey, P. R. "Different intermediate populations formed by tazobactam, sulbactam, and clavulanate reacting with SHV-1 b-lactamases: Raman crystallographic evidence" J. Am. Chem. Soc. 2009, 131, 2338-2347.

Pattanaik, P.; Bethel, C. R.; Hujer, A. M.; Hujer, K. M.; Distler, A. M.; Taracila, M.; Anderson, V. E.; Fritsche, T. R.; Jones, R. N.; Pagadala, S. R. R.; van den Akker, F.; Buynak, J. D.; Bonomo, R. A.  "Strategic Design of an Effective b-Lactamase Inhibitor: LN-1-255, A 6-alkylidene--2'-substituted penicillin sulfone." J. Biol. Chem.  2009,  284,  945-953.

Totir, M. A.; Cha, J.; Ishiwata, A.; Wang, B.; Sheri, A.; Anderson, V. E.; Buynak, J.; Mobashery, S.; Carey, P. R.  "Why Clinically Used Tazobactam and Sulbactam Are Poor Inhibitors of OXA-10 b-Lactamase: Raman Crystallographic Evidence." Biochemistry 2008, 47, 4094-4101.

Buynak, J. D. "Cutting and Stitching: the Cross-Linking of Peptidoglycan in the Assembly of the Bacterial Cell Wall." ACS Chem. Biol. 2007, 2, 602-605.

Kalp, M.; Sheri, A.; Buynak, J. D.; Bethel, C. R.; Bonomo, R. A. Carey, P. R. "Efficient Inhibition of Class A and Class D b-Lactamases by Michaelis Complexes." J. Biol. Chem. 2007, 282, 21588-21591.

Buynak, J. D.; Chen, H. "Cephalosporin-derived mercaptans as inhibitors of serine and metallo-b-lactamases." U. S. Patent 2006, US 2006178357 A1 200660810.

  • Padayatti, P. S.; Sheri, A.; Totir, M. A.; Helfand, M. S.; Carey, M. P.; Anderson, V. E.; Carey, P. R.; Bethel, C. R.; Bonomo, R. A.; Buynak, J. D.; van den Akker, F. "Rational Design of a b-Lactamase Inhibitor Achieved via Stabilization of the trans-Enamine Intermediate: 1.28 Å Crystal Structure of wt SHV-1 with a Penam Sulfone.  J. Am. Chem. Soc. 2006, 128, 13235-13242.

  • Buynak, John D.  “Understanding the longevity of the b-lactam antibiotics and of antibiotic/b-lactamase inhibitor combinations.”    Biochemical Pharmacology  2006,  71,  930-940. 

  • Zhu, D.; Yang, Y.; Buynak, J. D.; Hua, L. "Stereoselective ketone reduction by a carbonyl reductase from Sporobolomyces salmonicolor.  Substrate specificity, enantioselectivity and enzyme-substrate docking studies." Organic and Biomolecular Chemistry 2006, 4, 2690-2695.

  • Buynak, John D.; Ghadhachanda, Venkat R; Vogeti, Lakshminarayana; Zhang, H.; Chen, H. "Synthesis and Evaluation of 3-(Carboxymethylidene)- and 3-(Carboxymethyl)penicillinates as Inhibitors of b-Lactamase" Journal of Organic Chemistry 2005, 70, 4510-4513.

  • Hujer, A. M.; Kania, M.; Gerken, T.; Anderson, V. E.; Buynak, J.; Ge, X.; Caspers, P.; Page, M. G. P.; Rice, L. B.; Bonomo, R. A. "Structure-Activity Relationships of Different b-Lactam Antibiotics against a Soluble Form of Enterococcus faecium PBP5, a Type II Bacterial Transpeptidase"  Antimicrobial Agents and Chemotherapy 2005, 49, 612-618.

  • Buynak, J. D.; Chen H., Vogeti, L.; Gadhachanda, V. R.; Buchanan, C. A.; Palzkill, T.; Shaw, R. W.; Spencer, J.; Walsh, T. R. "Penicillin-derived Inhibitors that simultaneously target both metallo- and serine-b-Lactamases" Bioorganic & Medicinal Chemistry Letters 2004, 14, 1299-1304.

  • Buynak, John D.  "The discovery and development of modified penicillin- and cephalosporin-derived b-Lactamase inhibitors."    Current Medicinal Chemistry  2004,  11,  1951-1964.

Courses Taught:

  • Advanced Organic Chemistry (CHEM 5393)
  • Medicinal Chemistry (CHEM 5398)
  • Introduction to Bioorganic and Medicinal Chemistry (CHEM 6116)
  • Synthetic Strategies (CHEM 6119)
  • Chemical Mechanisms (CHEM 6130)