Faculty & Research

Svetlana N. Radyuk, Ph.D. Research Assistant Professor Ph.D.: Research Institute for Applied Microbiology, Russia Postdoctoral training: Southern Methodist University

Office: 340-DLS Tel: 214.768.2892 Fax: 214.768.3955 Email: snradyuk@smu.edu Lab: 317-DLS Tel: 214.768.2065

Research Interests

Innate Immunity in Drosophila/ Oxidative Stress and Immunity

The main goal of the research in my group is to understand the molecular mechanisms governing the regulation of innate immunity using Drosophila as a model and to determine whether enhanced immunity is a longevity determinant. In particular, the study is focused on determining the role of genes of antioxidative network in protecting cells against oxidative insult and in modulating the immune system.

Redox Signaling Diagram

Produced during metabolic reactions, inflammation, and phagocytosis, reactive oxygen and nitrogen species (ROS and RNS) not only cause tissue damage, but also act as messengers in signaling pathways, including modulation of immune responses.

The ROS/RNS concentrations are regulated by a vast array of different antioxidants that not only protect biomolecules from oxidative damage, but also function in mediating signal transduction, apparently by maintaining the “correct” levels of ROS/RNS.

Drosophila Immune Signaling Pathways

Flies respond to infection with both humoral and cellular immune defenses, including a powerful set of inducible peptide antibiotics – antimicrobial peptides (AMP). The induction of AMPs is regulated by both imd/Relish and Toll/Dif signaling pathways, although the specific molecular mechanisms of this induction have not been fully clarified. Moreover, the regulation of this system during aging is poorly understood.

We am testing a hypothesis that antioxidant enzymes, specifically, peroxiredoxins, i) are involved in eliciting the immune response by virtue of their peroxidase activity or by other molecular mechanisms and ii) whether the effects of aging influence the regulation of the immune response. To address these questions, we are using a wide range of molecular biology and genetics techniques.

Another avenue of my research is to determine variations in gene expression levels in response to different stresses, including those caused by toxic substances. The identification of biomarkers of such a response will facilitate the design of diagnostic detection systems and will suggest therapeutic strategies to alleviate toxicity.

Selected Publications

Orr, W.C., S.N. Radyuk, L. Prabhudesai, D. Toroser, J.J. Benes, J.M. Luchak, R.J. Mockett, I. Rebrin, J.G. Hubbard, and R.S. Sohal. 2005. Overexpression of glutamate-cysteine ligase extends life span in Drosophila melanogaster. J Biol Chem. epub ahead of press.

Radyuk, S.N., Klichko, V.I. and Orr, W.C. (2004) Profiling Cu,Zn-superoxide dismutase in Drosophila melanogaster - a critical regulatory role for intron/exon sequence within the coding domain. Gene. 328, 37-48.

Klichko, V.I., Radyuk, S.N. and Orr, W.C. (2004) Profiling catalase gene expression in Drosophila melanogaster during development and aging. Archives of Insect Biochemistry and Physiology. 56, 34-50.

Radyuk, S.N., Mericko, P.A., Popova, T.G., Grene, E., Alibek, K. In vitro-generated respiratory mucosa: a new tool to study inhalational anthrax. Biochem. Biophys. Res. Commun. 2003 Jun 6;305(3):624-32.

Radyuk S.N., Sohal R.S., Orr W.C. (2003). Thioredoxin peroxidases can foster cytoprotection or cell death in response to different stressors - a study of thioredoxin peroxidase under- and over-expression in Drosophila cells. Biochem J. 2003 May 1;371(Pt 3):743-52.

Mockett R.J., Radyuk S.N., Benes J.J., Orr W.C., Sohal R.S. (2003). Phenotypic effects of familial amyotrophic lateral sclerosis mutant Sod alleles in transgenic Drosophila. Proc Natl Acad Sci U S A 2003 Jan 7;100(1):301-6

Radyuk, S.N., Klichko, V.I., Spinola, B., Sohal, R.S., Orr, W.C. (2001). The peroxiredoxin gene family in Drosophila melanogaster. Free Radic. Biol. Med., Nov 1;31(9):1090-100

Radyuk, S.N., Klichko, V.I., Orr, W.C. (2000). Catalase expression in Drosophila melanogaster is responsive to ecdysone and exhibits both transcriptional and post-transcriptional regulation. Arch Insect Biochem Physiol Oct;45(2):79-93

Klichko, V.I., Radyuk, S.N., Orr, W.C. (1999). CuZn-SOD promoter-driven expression in the Drosophila central nervous system. Neurobiol Aging Sep-Oct;20(5):537-43

Mockett RJ, Orr WC, Rahmandar JJ, Benes JJ, Radyuk SN, Klichko VI, Sohal RS (1999). Overexpression of Mn-containing superoxide dismutase in transgenic Drosophila melanogaster. Arch Biochem Biophys Nov 15;371(2):260-9

Candas M., Sohal R.S., Radyuk S.N., Klichko V.I., Orr W.C. (1997). Molecular Organization of the Glutathione Reductase Gene in Drozophila Melanogaster. Arch.Biochem.Bioph.339: 323-334

Radiuk S.N., Ryzhov, K.A., Matsevich, G.R. (1998). Polymerase chain reaction in the diagnosis of tuberculosis. Zh Mikrobiol Epidemiol Immunobiol. May-Jun;(3):95-8. Review.

Radiuk S.N.,Matsevich, G.R. (1997). Polymerase chain reaction in the diagnosis of tuberculosis. Klin Lab Diagn. Jul;(7):11-3

Matsevich G.R., Radiuk S.N., Ryzhov K.A., Andzhaparidze O.G. (1996). Evolution of methods of laboratory diagnosis of orthopoxvirus infection. Vopr. Virusol. 41: 195-197

Radiuk S.N., Matsevich G.R., Ryzhov K.A., Lariukova T.A. (1996). Nonisotopic variant of quantitative analysis using polymerase chain reaction for diagnosing HIV infection. Vopr. Virusol. 41: 44-45

Radiuk S.N., Matsevich G.R., Andzhaparidze O.G. (1994). A nonisotopic method of quantitative PCR analysis in diagnosing HIV infection. Vopr. Virusol. 39: 242-245

Radiuk S.N., Matsevich G.R., Andzhaparidze O.G. (1994). The polymerase chain reaction in the diagnosis and prognosis of HIV infection. Vopr. Virusol. 39: 98-101. Review

Radiuk S.N., Matsevich G.R., Tarantul V.Z., Andzhaparidze O.G.(1994). Primers for the nef gene in the diagnosis of HIV infection using The polymerase chain reaction. Vopr. Virusol. 39: 53-56

Slepushkin V.A., Kornilaeva G.V., Andreev S.M., Sidorova M.V., Petrukhina A.O.,Matsevich G.R., Raduk S.N., Grigoriev V.B., Makarova T.V., Lukashov V.V., Karamov E.V. (1993). Inhibition of human immunodeficiency virus type 1 (HIV-1) penetration into target cells by synthetic peptides mimicking the N-terminus of the HIV-1 transmembrane glycoprotein. Virology 194: 294-301

Support

R21 01/01/05-12/31/06 (Principal Investigator) Funded by National Institutes of Health, Direct cost $125000 per year
The The Role of peroxiredoxins in Immunity and Aging

SBIR 08/16/04-12/16/05 (Sub-Contract) Funded by US Airforce; Direct cost $75000 for 16 months
Automated Individual Real-Time Toxic Exposure Monitoring (AIRTEM) System

Education

1979:	MS Chemistry, M.V.Lomonosov Moscow State University, Russia.
1990:	PhD Biology Research Institute for Applied Microbiology, Russia.

Professional Experience

2003-present	Research Assistant Professor, Southern Methodist University, Dallas, TX.
2002-2003:	Senior Scientist, Advanced Biosystems, Inc., Manassas, VA. Affiliate Professor, George Mason University, Manassas, VA.
1996-2002:	Postdoctoral Research Associate, Southern Methodist University, Dallas, TX.
1991-1996:	Principal Research Scientist, Research Institute for Viral Preparations, Moscow, Russia.
1982-1991:	Junior Research Scientist, Research Institute of Applied Microbiology, Obolensk, Russia.
1979-1982:	Engineer, Research Institute of Applied Chemistry, Zagorsk, Russia.